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Effect of biochar amendment on soil’s retention capacity for estrogenic hormones from poultry manure treatment

Sukhjot MANN, Zhiming QI, Shiv O. PRASHER, Lanhai LI, Dongwei GUI, Qianjing JIANG

《农业科学与工程前沿（英文）》 2017年 第4卷 第2期   页码 208-219 doi: 10.15302/J-FASE-2017138

摘要： Most animals, including humans, produce natural sex hormones such as estrogens: 17β-estradiol (E2) and estrone (E1). These compounds are able to disrupt the reproductive systems of living organisms at trace concentrations (ng·L ). This experiment tests the hypothesis that 1% slow pyrolysis biochar-amended sandy soil could retain significant amount of estrogens (E1, E2) from poultry manure in its second year of application. The experiment was conducted over 46 days and consisted of a series of lysimeters containing sandy soil with biochar-amended topsoil. The application rate of poultry manure was kept at 2.47 kg·m . The biochar held a significant concentration of hormone during the first year of its application. However, in the following year (current study), there was no significant retention of hormones in the biochar-amended soil. During the first year after application, the biochar was fresh, so its pores were available for hydrophobic interactions and held significant concentration of hormones. As time passed there were several biotic and abiotic changes on the surface of the biochar so that after some physical fragmentation, pores on the surface were no longer available for hydrophobic interactions. The biochar started releasing dissolved organic carbon, which facilitated greater mobility of hormones from poultry manure down the soil profile.

关键词： adsorption     degradation     dissolved organic carbon     17β-estradiol (E2)     estrone (E1)    

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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

《农业科学与工程前沿（英文）》 2021年 第8卷 第2期

摘要：

Drought stress typically causes heavy losses in apple production and uncovering the mechanisms by which apple tolerates drought stress is important in apple breeding. MdSIZ1 is a SUMO (small ubiquitin-like modifier) E3 ligase that promotes SUMO binding to substrate proteins. Here, we demonstrate that MdSIZ1 in apple has a negative relationship with drought tolerance. MdSIZ1 RNAi transgenic apple trees had a higher survival rate after drought stress. During drought stress they had higher leaf water potential, reduced ion leakage, lower H2O2 and malondialdehyde contents, and higher catalase activity. In addition, MdSIZ1 RNAi transgenic plants had a higher net photosynthetic rate during the latter period of drought stress. Finally, the transgenic apple trees also altered expression levels of some microRNAs in response to drought stress. Taken together, these results indicate that apple MdSIZ1 negatively regulates drought stress by enhancing leaf water-holding capacity and antioxidant enzyme activity.

 

关键词： apple / drought tolerance / gene expression / MdSIZ1    

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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 662-662 doi: 10.15302/J-FASE-2021408

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Role of water chemistry on estrone removal by nanofiltration with the presence of hydrophobic acids

Xue JIN,Jiangyong HU

《环境科学与工程前沿（英文）》 2015年 第9卷 第1期   页码 164-170 doi: 10.1007/s11783-014-0747-9

摘要： Hydrophobic acid organic matter (HpoA) extracted from treated effluent has been known to improve the rejection of steroid hormone estrone by reverse osmosis (RO) and nanofiltration (NF) membranes. In this study, the effects of solution chemistry (solution pH and ionic strength) on the estrone rejection by NF membrane with the presence of HpoA were systematically investigated. Crossflow nanofiltration experiments show that the presence of HpoA significantly improved estrone rejection at all pH and ionic strength levels investigated. It is consistently shown that the “enhancement effect” of HpoA on estrone rejection at neutral and alkaline pH is attributed to the binding of estrone by HpoA macromolecules via hydrogen bonding between phenolic functional groups in feed solutions, which leads to an increase in molecular weight and appearance of negative charge. The membrane exhibited the best performance in terms of estrone rejection at pH 10.4 (compared to pH 4 and pH 7) as a result of strengthening the electrostatic repulsion between estrone and membrane with the presence of HpoA. At neutral pH level, the ability of HpoA macromolecules to promote estrone rejection became stronger with increasing ionic strength due to their more extended conformation, which created more chances for the association between estrone and HpoA. The important conclusion of this study is that increasing solution pH and salinity can greatly intensify the “enhancement effect” of HpoA. These results can be important for NF application in direct/indirect potable water reuse.

关键词： indirect potable water reuse     steroid hormone     nanofiltration(NF)     rejection     water chemistry     hydrophobic acids    

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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

Baohua CHU, Jia SUN, Huan DANG, Ziqing MA, Shuang ZHAO, Qingmei GUAN, Xuewei LI

《农业科学与工程前沿（英文）》   页码 247-261 doi: 10.15302/J-FASE-2021388

摘要： Drought stress typically causes heavy losses in apple production and uncovering the mechanisms by which apple tolerates drought stress is important in apple breeding. MdSIZ1 is a SUMO (small ubiquitin-like modifier) E3 ligase that promotes SUMO binding to substrate proteins. Here, we demonstrate that in apple has a negative relationship with drought tolerance. RNAi transgenic apple trees had a higher survival rate after drought stress. During drought stress they had higher leaf water potential, reduced ion leakage, lower H O and malondialdehyde contents, and higher catalase activity. In addition, RNAi transgenic plants had a higher net photosynthetic rate during the latter period of drought stress. Finally, the transgenic apple trees also altered expression levels of some microRNAs in response to drought stress. Taken together, these results indicate that apple MdSIZ1 negatively regulates drought stress by enhancing leaf water-holding capacity and antioxidant enzyme activity.

关键词： apple     drought tolerance     gene expression     MdSIZ1    

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Rdh13 deficiency weakens carbon tetrachloride-induced liver injury by regulating Spot14 and Cyp2e1 expression

Xiaofang Cui, Benting Ma, Yan Wang, Yan Chen, Chunling Shen, Ying Kuang, Jian Fei, Lungen Lu, Zhugang Wang

《医学前沿（英文）》 2019年 第13卷 第1期   页码 104-111 doi: 10.1007/s11684-017-0568-x

摘要： Mitochondrion-localized retinol dehydrogenase 13 (Rdh13) is a short-chain dehydrogenase/reductase involved in vitamin A metabolism in both humans and mice. We previously generated knockout mice and showed that Rdh13 deficiency causes severe acute retinal light damage. In this study, considering that Rdh13 is highly expressed in mouse liver, we further evaluated the potential effect of Rdh13 on liver injury induced by carbon tetrachloride (CCl ). Although Rdh13 deficiency showed no significant effect on liver histology and physiological functions under regular culture, the mice displayed an attenuated response to CCl -induced liver injury. Their livers also exhibited less histological changes and contained lower levels of liver-related metabolism enzymes compared with the livers of wild-type (WT) mice. Furthermore, the mice had Rdh13 deficiency and thus their liver cells were protected from apoptosis, and the quantity of their proliferative cells became lower than that in WT after CCl exposure. The ablation of gene decreased the expression levels of thyroid hormone-inducible nuclear protein 14 (Spot14) and cytochrome P450 (Cyp2e1) in the liver, especially after CCl treatment for 48 h. These data suggested that the alleviated liver damage induced by CCl in mice was caused by Cyp2e1 enzymes, which promoted reductive CCl metabolism by altering the status of thyroxine metabolism. This result further implicated Rdh13 as a potential drug target in preventing chemically induced liver injury.

关键词： retinol dehydrogenase 13     carbon tetrachloride     acute liver injury     Cyp2e1     Spot14    

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中国的探月工程——中国航天第三个里程碑

栾恩杰

《中国工程科学》 2006年 第8卷 第10期   页码 31-36

摘要：

人类对月球的探测显示了人类的智慧和能力，也带动了人类社会及科学技术的进步和发展。中国以“绕”、“落”、“回”为标志的探月工程是中国航天的第三个里程碑性工程，既是国力强大的体现，也是中国航天能力的表征。

关键词： 中国航天     月球勘探     嫦娥1号    

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Genetic variants in the ADD1 and GNB3 genes and blood pressure response to potassium supplementation

Collaborative Research Group, De-Pei LIU PhD, Lai-Yuan WANG PhD, Jing CHEN MD, MSc, Jiang HE MD, PhD, Cashell E.RAO PhD, Charles GU PhD, James E. HIXSON PhD, Chung-Shiuan CHEN MS8, Paul K.

《医学前沿（英文）》 2010年 第4卷 第1期   页码 59-66 doi: 10.1007/s11684-010-0015-8

摘要： Dietary potassium-supplementation has been associated with a decreased risk of hypertension and other cardiovascular outcomes. However, blood pressure (BP) responses to potassium supplementation vary among individuals. This study was designed to examine the association between 12 single nucleotide polymorphisms (SNPs) in the adducin 1 alpha (ADD1) and guanine nucleotide binding protein (G protein) beta polypeptide 3 (GNB3) genes and systolic BP (SBP), diastolic BP (DBP), and mean arterial pressure (MAP) responses to potassium-supplementation. We conducted a 7-day high-sodium intervention (307.8 mmol sodium/day) followed by a 7-day high-sodium with potassium-supplementation (60 mmol potassium/day) among 1906 Han Chinese participants from rural north China. BP measurements were obtained at the end of each intervention period using a random-zero sphygmomanometer. We identified significant associations between ADD1 variant rs17833172 and SBP, DBP, and MAP responses to potassium-supplementation (all <0.0001) that remained significant after adjustment for multiple comparisons. In participants that were heterozygous or homozygous for the G allele of this marker, SBP, DBP, and MAP response to potassium-supplementation were −3.52 (−3.82, −3.21), −1.41 (−1.66, −1.15) and −2.12 (−2.37, −1.87), respectively, as compared to the corresponding responses of 1.99 (0.25, 3.73), −0.65 (−0.10, −0.21), and −0.23 (−0.37, 0.83), respectively, for those who were homozygous for A allele. In addition, participants with at least one copy of the G allele of rs12503220 of the ADD1 gene had significantly increased DBP and MAP response to potassium-supplementation ( = 0.0041 and 0.01, respectively), which was also significant after correction for multiple testing. DBP and MAP responses to potassium-supplementation were −1.36 (−1.63, −1.10) and −2.07 (−2.32, −1.82) for those with at least G allele compared to corresponding responses of 0.86 (−0.68, 2.40) and −0.45 (−1.74, 0.84) for those who were homozygous for A allele. In summary, our study identified novel associations between genetic variants of the ADD1 gene and BP response to potassium-supplementation, which could have important clinical and public health implications. Future studies aimed at replicating these novel findings are warranted.

关键词： blood pressure     genetics     polymorphism     die-tary potassium     potassium sensitivity     adducin 1 alpha (ADD1)     guanine nucleotide binding protein beta polypeptide 3 (GNB3)    

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mTOR-targeted cancer therapy: great target but disappointing clinical outcomes, why?

Shi-Yong Sun

《医学前沿（英文）》 2021年 第15卷 第2期   页码 221-231 doi: 10.1007/s11684-020-0812-7

摘要： The mammalian target of rapamycin (mTOR) critically regulates several essential biological functions, such as cell growth, metabolism, survival, and immune response by forming two important complexes, namely, mTOR complex 1 (mTORC1) and complex 2 (mTORC2). mTOR signaling is often dysregulated in cancers and has been considered an attractive cancer therapeutic target. Great efforts have been made to develop efficacious mTOR inhibitors, particularly mTOR kinase inhibitors, which suppress mTORC1 and mTORC2; however, major success has not been achieved. With the strong scientific rationale, the intriguing question is why cancers are insensitive or not responsive to mTOR-targeted cancer therapy in clinics. Beyond early findings on induced activation of PI3K/Akt, MEK/ERK, and Mnk/eIF4E survival signaling pathways that compromise the efficacy of rapalog-based cancer therapy, recent findings on the essential role of GSK3 in mediating cancer cell response to mTOR inhibitors and mTORC1 inhibition-induced upregulation of PD-L1 in cancer cells may provide some explanations. These new findings may also offer us the opportunity to rationally utilize mTOR inhibitors in cancer therapy. Further elucidation of the biology of complicated mTOR networks may bring us the hope to develop effective therapeutic strategies with mTOR inhibitors against cancer.

关键词： mTOR     cancer therapy     resistance     GSK3     protein degradation     E3 ubiquitin ligase     PD-L1    

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Effects of hypoxia inducible factor-1alpha siRNA on the invasion of human Hela cells and expression of

Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,

《医学前沿（英文）》 2009年 第3卷 第3期   页码 303-308 doi: 10.1007/s11684-009-0060-3

摘要： The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible factor-1α (HIF-1α) were investigated. The Hela cells were divided into three groups, namely, H (non-transfected Hela cells), H (pGenesil-1 empty plasmid-transfected Hela cells), and H (HIF-1α-shRNA plasmid-transfected Hela cells), and were cultured under hypoxia (1% O) and normoxia for 48h. The expression of HIF-1α, E-cadherin, β-catenin, and actin was detected using Western blot. The scratch test and the invasion assay were applied to examine the invasion in each group. The expression of HIF-1α, E-cadherin, and β-catenin in tumor grafts was assayed immunohistochemically. Western blot results revealed that the bands of HIF-1α, E-cadherin, β-catenin, and actin proteins were detected in the H and H groups under hypoxia for 48h. The expression of E-cadherin, β-catenin, and actin was detected in the H group under hypoxia for 48h, and normoxia. In the H, H, and H groups under normoxia, and the H group under hypoxia for 48h, no expression of HIF-1α was detectable. The scratch test showed that the invasive ability in the H group was significantly alleviated. Immunohistochemically, it was found that there was a significant difference in the expression of HIF-1α, E-cadherin, and β-catenin between the H and H groups (<0.05), but the difference was not significant between the H and H groups. It was concluded that the effects of hypoxia on the invasion of human cervical cancer Hela cells and the expression of related proteins (E-cadherin, β-catenin, and actin) depend on HIF-1α.

关键词： hypoxia     actin     β     -catenin     E-cadherin     invasion    

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Heterologous expression of

Potter,Francis E. Nano

《环境科学与工程前沿（英文）》 2017年 第11卷 第2期 doi: 10.1007/s11783-017-0910-1

摘要： Maximum growth rate of mutant was 0.083 h with 5% CO . Maximum biomass concentration of mutant was 3.697 g·L . mutant can tolerate gas aeration with 15% CO . Maximum specific activity of laminarinase was 4.325 U·mg dry mass. Optimal pH and temperature of laminarinase activity were 8.0 and 70°C. The gene for the catalytic domain of thermostable endo-β-1,3-glucanase (laminarinase) LamA was cloned from Thermotoga maritima MSB8 and heterologously expressed in a bioengineered Synechococcus sp. PCC 7002. The mutant strain was cultured in a photobioreactor to assess biomass yield, recombinant laminarinase activity, and CO2 uptake. The maximum enzyme activity was observed at a pH of 8.0 and a temperature of 70°C. At a CO2 concentration of 5%, we obtained a maximum specific growth rate of 0.083 h , a biomass productivity of 0.42 g·L ·d , a biomass concentration of 3.697 g·L , and a specific enzyme activity of the mutant strain of 4.325 U·mg dry mass. All parameters decreased as CO2 concentration increased from 5% to 10% and further to 15% CO2, except enzyme activity, which increased from 5% to 10% CO2. However, the mutant culture still grew at 15% CO2 concentration, as reflected by the biomass productivity (0.26 g·L ·d ), biomass concentration (2.416 g·L ), and specific enzyme activity (3.247 U·mg dry mass).

关键词： Synechococcus sp. PCC 7002     Thermotoga maritima     LamA gene     Endo-β-1     3-glucanase     CO2 fixation    

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Combined gemcitabine and CHK1 inhibitor treatment induces apoptosis resistance in cancer stem cell-like

null

《医学前沿（英文）》 2013年 第7卷 第4期   页码 462-476 doi: 10.1007/s11684-013-0270-6

摘要：

Evaluating the effects of novel drugs on appropriate tumor models has become crucial for developing more effective therapies that target highly tumorigenic and drug-resistant cancer stem cell (CSC) populations. In this study, we demonstrate that a subset of cancer cells with CSC properties may be enriched into tumor spheroids under stem cell conditions from a non-small cell lung cancer cell line. Treating these CSC-like cells with gemcitabine alone and a combination of gemcitabine and the novel CHK1 inhibitor PF-00477736 revealed that PF-00477736 enhances the anti-proliferative effect of gemcitabine against both the parental and the CSC-like cell populations. However, the CSC-like cells exhibited resistance to gemcitabine-induced apoptosis. Collectively, the spheroid-forming CSC-like cells may serve as a model system for understanding the mechanism underlying the drug resistance of CSCs and for guiding the development of better therapies that can inhibit tumor growth and eradicate CSCs.

关键词： drug resistance     cancer stem cell     checkpoint kinase 1 (CHK1)     PF-00477736     lung cancer     tumorigenicity    

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Sustainability of metal recovery from E-waste

Biswajit Debnath, Ranjana Chowdhury, Sadhan Kumar Ghosh

《环境科学与工程前沿（英文）》 2018年 第12卷 第6期 doi: 10.1007/s11783-018-1044-9

摘要：

Metal recovery techniques from electronic waste reported in literature.

Metal recovery processes followed in Industries from electronic waste.

Sustainability analysis of metal recovery processes from electronic waste.

关键词： E-waste     Metal recovery     Metal Recovery from E-waste (MREW)     Sustainability    

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有限域上Galois型非线性移位寄存器的能观性 Research Article

高哲1,冯俊娥1,于永渊1,崔彦君2

《信息与电子工程前沿（英文）》 2022年 第23卷 第10期   页码 1533-1545 doi: 10.1631/FITEE.2200228

摘要：

能观性可以确保任何两个不同初始状态都可以由它们的输出序列唯一确定，因此流密码必须避免不可观的非线性反馈移位寄存器，以防止等效密钥的出现。本文讨论了有限域上Galois型非线性反馈移位寄存器的能观性。通过半张量积，Galois型非线性反馈移位寄存器可被视为逻辑网络。本文介绍了状态转移矩阵的向量形式，据此提出一个充分必要条件以及判定一般Galois型非线性反馈移位寄存器能观性的算法。此外，本文定义了一个新的能观性矩阵，通过该矩阵可推导出计算复杂度较低的矩阵方法。此外，研究两种特殊类型的Galois型非线性反馈移位寄存器的能观性：全长Galois型非线性反馈移位寄存器和非奇异Galois型非线性反馈移位寄存器。提出两种方法确定这两种特殊类型的非线性反馈移位寄存器的能观性，并提供一些数值示例支持这些结果。

关键词： 能观性；非线性反馈移位寄存器（NFSRs）；Galois型非线性反馈移位寄存器；半张量积；有限域；逻辑网络    

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risks of heavy metals, polybrominated diphenyl ethers, and polychlorinated dibenzo-dioxins/furans at e-waste

《环境科学与工程前沿（英文）》 2023年 第17卷 第7期 doi: 10.1007/s11783-023-1679-z

摘要：

● Heavy metals and organic toxins may persist in legacy sites for a long time.

关键词： E-waste     Human health risk     Organ risk     Heavy metal toxicity     PBDE     PCDD/F    

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标题 作者 时间 类型 操作

Effect of biochar amendment on soil’s retention capacity for estrogenic hormones from poultry manure treatment

Sukhjot MANN, Zhiming QI, Shiv O. PRASHER, Lanhai LI, Dongwei GUI, Qianjing JIANG

期刊论文

APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

期刊论文

APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

期刊论文

Role of water chemistry on estrone removal by nanofiltration with the presence of hydrophobic acids

Xue JIN,Jiangyong HU

期刊论文

APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

Baohua CHU, Jia SUN, Huan DANG, Ziqing MA, Shuang ZHAO, Qingmei GUAN, Xuewei LI

期刊论文

Rdh13 deficiency weakens carbon tetrachloride-induced liver injury by regulating Spot14 and Cyp2e1 expression

Xiaofang Cui, Benting Ma, Yan Wang, Yan Chen, Chunling Shen, Ying Kuang, Jian Fei, Lungen Lu, Zhugang Wang

期刊论文

中国的探月工程——中国航天第三个里程碑

栾恩杰

期刊论文

Genetic variants in the ADD1 and GNB3 genes and blood pressure response to potassium supplementation

Collaborative Research Group, De-Pei LIU PhD, Lai-Yuan WANG PhD, Jing CHEN MD, MSc, Jiang HE MD, PhD, Cashell E.RAO PhD, Charles GU PhD, James E. HIXSON PhD, Chung-Shiuan CHEN MS8, Paul K.

期刊论文

mTOR-targeted cancer therapy: great target but disappointing clinical outcomes, why?

Shi-Yong Sun

期刊论文

Effects of hypoxia inducible factor-1alpha siRNA on the invasion of human Hela cells and expression of

Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,

期刊论文

Heterologous expression of

Potter,Francis E. Nano

期刊论文

Combined gemcitabine and CHK1 inhibitor treatment induces apoptosis resistance in cancer stem cell-like

null

期刊论文

Sustainability of metal recovery from E-waste

Biswajit Debnath, Ranjana Chowdhury, Sadhan Kumar Ghosh

期刊论文

有限域上Galois型非线性移位寄存器的能观性

高哲1,冯俊娥1,于永渊1,崔彦君2

期刊论文

risks of heavy metals, polybrominated diphenyl ethers, and polychlorinated dibenzo-dioxins/furans at e-waste

期刊论文